Fig. 8

Hematopoietic differentiation bias of Naïve-like H9. a, b Violin plots show the expression level distributions of mesendoderm genes (T, FGF4, MIXL, GSC, FOXA2, EOMES, GATA4, and LEFTY1) (a) and neural genes (ALCAM, OLFM1, SIGMAR1, DPYSL3, CPNE1, KCNQ2, BEX1, and STMN3) (b). c Flow cytometry analysis of hematopoietic progenitors derived from hPSCs. Significant difference was assessed by the t-test. ***p < 0.001, **p < 0.01, *p < 0.05. d The morphology and number of hematopoietic CFUs. Scale bars = 100 μm. e Western blot analysis of MAPK (ERK1/2, JNK, and P38) in naïve and primed H9