Fig. 5

Wnt + induced cell state transition is contact-dependent. a Appearance of typical WT cells and K14ΔNβ-cateninER co-cultures stained for OPP. b Left: Quantification of OPP in K14ΔNβ-cateninER cells and their neighbours in control and 4OHT treated conditions. Right: Similar quantification for cells co-cultured in low calcium medium (0.1 mM) to inhibit cell–cell contact (n = 3 independent cultures, n = 3 technical replicates, all pooled). c Appearance of typical WT cells and K14ΔNβ-cateninER co-cultures stained for Smad4. d Left: Quantification of Smad4 in K14ΔNβ-cateninER cells and their neighbours in control and 4OHT treated conditions. Right: Similar quantification for cells co-cultured in low calcium medium (0.1 mM) to inhibit cell–cell contact (n = 3 independent cultures, n = 3 technical replicates, all pooled). All scale bars, 20 μm, n.s. not significant