Fig. 4

The down-regulation of mut-p53, YAP, TAZ, and TEAD affects circPVT1 but not PVT1 expression. a–g CAL27 cell line. a p53 mRNA level was detected by RT-qPCR 24 and 48 h after transfection with siRNAp53 or siRNAct. b circPVT1 level was detected by RT-qPCR 24 and 48 h after transfection with siRNAp53 or siRNAct. c PVT1 level was detected by RT-qPCR 24 and 48 h after transfection with siRNAp53 or siRNAct. d YAP mRNA level was detected by RT-qPCR 48 h after transfection with siRNA-YAP or siRNAct. e TAZ mRNA level was detected by RT-qPCR 48 h after transfection with siRNA-TAZ or siRNAct. f TEAD1 mRNA level was detected by RT-qPCR 48 h after transfection with siRNA-TEAD1-4 or in siRNAct. g circPVT1 and PVT1 levels were detected by RT-qPCR 48 h after transfection with siRNA-YAP, siRNA-TAZ, or siRNA-TEAD1-4. h–k A253 cell line. h p53 mRNA level was detected by RT-qPCR 48 h after transfection. i YAP mRNA level was detected by RT-qPCR 48 h after transfection. j circPVT1 level was detected by RT-qPCR 48 h after transfection. k PVT1 level was detected by RT-qPCR 48 h after transfection. The transfections were performed using the siRNAs and the vectors indicated in the graphs. Data are shown as mean of three replicates ± standard deviation (Student’s test; *p < 0.05; **p < 0.01; ***p < 0.001)