Fig. 3

Validating BayFish on synthetic smFISH data. a Example of a technical replicate of synthetic smFISH data generated for an underlying k ₁-stimulus model, where n=100 cells per time point were sampled from the mRNA and active transcription site distribution (solid lines). Our synthetic data were sampled at t=0,5,15,25 min after induction. b Marginal Bayesian posterior distributions of parameters estimated by BayFish on three technical replicates (different colors). c Marginal Bayesian posterior distributions of parameters estimated by BayFish for different sampling densities (n=30,100,300,1000 cells per time point) of the same technical replicate. Vertical dashed lines are the true parameters of the k ₁-stimulus model used to generate the synthetic data