Fig. 4

Detection of differential splicing between cells. a Percentage of differential splicing events between human HCT116 cells, detected by MISO, rMATS, BRIE, and its mode with shared weights (i.e., BRIE.share) with different thresholds. MISO and BRIE use the Bayes factor (BF) and rMATS uses false discovery rate (q value). b Percentage of differential splicing events between mouse early embryonic cells at day 6.5 or day 7.75. The threshold is BF>10 for MISO and BRIE, and q<0.05 for rMATS. The diamond indicates pooled reads of 20 cells in each group. c An example exon-skipping event in DNMT3B in three mouse cells at 6.5 days and three cells at 7.75 days. The left panel is a sashimi plot of the read density and the number of junction reads. The right panel shows the prior distribution as a blue curve and a histogram of the posterior distribution in black, both learned by BRIE. For the histogram, the red line is the mean and the dashed lines are the 95 % confidence interval. BF Bayes factor