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Fig. 4 | Genome Biology

Fig. 4

From: Insights into the design and interpretation of iCLIP experiments

Fig. 4

Non-coinciding cDNA-starts are a result of constrained cDNA-ends. a The cDNA-starts (solid lines) and cDNA-ends (dotted lines) of PTBP1-iCLIP1 are plotted around the cDNA-start peak that was identified within each of the 1000 > 30 nt long crosslink clusters that have the highest total cDNA count. cDNAs are divided into four length categories: 17–29 nt, 30–34 nt, 35–39 nt and >39 nt. The inner plot shows the empirical cumulative distribution from all four length categories in the region between –25 nt and 25 nt around cDNA-start peaks. b As in (a), but plotted around the cDNA-end peak that was identified within the 30 nt downstream of each of the 1000 > 30 nt long crosslink clusters that have the highest total cDNA count. The inner box plot shows the ratio of cDNA counts (log2) at the position 0 (overlapping with cDNA-end peak) compared to the average count of cDNAs in the region from 5 nt to 25 nt downstream of the cDNA-end peak (marked by horizontal arrow). c As in (a), but for PTBP1-iCLIP2. d As in (b), but for PTBP1-iCLIP2

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