Fig. 6

Upstream regulators of IBD candidate genes. a The charts show the upstream regulators of the identified IBD candidate genes in the separate cell types. Bars correspond with the –Log of the p value, the dashed line indicates the threshold for significance. The analyses were performed using Ingenuity’s Upstream Regulator Analysis (see “Methods” for more information). All significantly enriched upstream regulators can be found in Additional file 4: Table S5. b Relative RNA expression before (–) and upon (+) exposure of human intestinal organoids to microbial antigens. Data were normalized to β-ACTIN mRNA abundances. HNF4α and NF-κB are upregulated upon expose. HNF4α hepatocyte nuclear factor α, KAT6A K (lysine) acetyl transferase 6A, COMMD3-BMI1 naturally occurring read-through transcription between the neighboring COMM domain-containing protein 3 and polycomb complex protein BMI-1, ASXL1 additional sex combs like transcriptional regulator 1, TP53 tumor protein p53, IL4 Interleukin 4, CD40LG CD40 ligand, TCR T-cell receptor, GnRN gonadotropin releasing hormone, ESR1 estrogen receptor 1