Fig. 3

Time dynamics of the correlations between RNA and protein abundance. a The density of RNA:protein Spearman correlation coefficients (ρ) by time point, with ticks displaying individual genes from the three time points (0 hr = purple, 24 hr = green, 48 hr = blue). b Categorized by temporal correlation pattern, the correlations of the same gene across time points are juxtaposed. *, p value < 0.05; **, p value < 0.01. c, d Translational control of MET protein expression. c Approximate primer (red) locations for assays used to dissect splicing status of MET transcripts. RefSeq entries NM_000245.2 (short form) and NM_001127500.1 (long form) are the two reported splice isoforms of the MET transcript. The thinner gray bar indicates the segment included in the long form but not in the short form. Assay MET.1_R detects unspliced RNA; MET.2_R detects long form spliced RNA and, at reduced efficiency, unspliced RNA but was determined to not be quantitative by population lysate dilutions (Additional file 3: Figure S3b); MET.3_R detects unspliced RNA; MET.4_R detects long form spliced RNA and unspliced RNA at equal efficiency; MET.5_R detects short form spliced RNA and, at reduced efficiency, long form spliced RNA; MET.6_R detects both spliced forms and unspliced RNA with equal efficiency. d Distributions of spliced MET RNA (left) and MET protein at the three time points used in this study