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Fig. 7 | Genome Biology

Fig. 7

From: Mapping H4K20me3 onto the chromatin landscape of senescent cells indicates a function in control of cell senescence and tumor suppression through preservation of genetic and epigenetic stability

Fig. 7

Reintroduction of SUV420H2/H4K20me3 attenuates the proliferative capacity of SUV420H2/H4K20me3-deficent HT1080 tumor cells. a Western blot of SUV420H2 and β-actin from whole cell extracts of proliferating (PRO) and RS IMR90 cells and HT1080 cells. b Western blot of H4K20me3 and histone H4 from whole cell extracts of PRO and RS IMR90 cells and HT1080 cells. c SUV420H2 expression in various human cancers relative to the reference population (either all tumors that are diploid for the gene in question or, when available, normal adjacent tissue). Data obtained from the cBioPortal for Cancer Genomics. X-axis, cancer type: (1) acute myeloid leukemia, (2) acute myeloid leukemia, (3) bladder urothelial carcinoma, (4) bladder urothelial carcinoma, (5) brain lower grade glioma, (6) breast invasive carcinoma, (7) cervical squamous cell carcinoma and endocervical adenocarcinoma, (8) colon and rectum adenocarcinoma, (9) glioblastoma multiforme, (10) glioblastoma, (11) head and neck squamous cell carcinoma, (12) head and neck squamous cell carcinoma, (13) kidney chromophobe, (14) kidney renal clear cell carcinoma, (15) kidney renal clear cell carcinoma, (16) kidney renal papillary cell carcinoma, (17) liver hepatocellular carcinoma, (18) lung adenocarcinoma, (19) lung adenocarcinoma, (20) lung squamous cell carcinoma, (21) ovarian serous cystadenocarcinoma, (22) pancreatic adenocarcinoma, (23) prostate adenocarcinoma, (24) sarcoma, (25) skin cutaneous melanoma, (26) stomach adenocarcinoma, (27) thyroid carcinoma, (28) uterine corpus endometrioid carcinoma. Y-axis, difference in SUV420H2 expression (Z score, normal/cancer). d Western blot of indicated proteins from whole cell extracts of HT1080 cells infected with vector control (CON) or MYC-tagged SUV420H2 (H2). e CON and H2 HT1080 cells were pulse labeled with 5-BrdU, fixed, and stained with propidium iodide. Fluorescence activated cell sorting (FACS) analysis to determine cell cycle distribution based on propidium iodide. f FACS analysis of cells from e to determine proportion of cells in G1, S, and G2/M phases based on 5-BrdU and propidium iodide; error bars represent standard deviation (SD; n = 2). g Growth curves expressed as log cumulative cell number for CON and H2 HT1080 cells measured for 32 days after infection. h Mean volumes of tumors formed after subcutaneous injection of CON or H2 HT1080 cells into CD-1 nude mice (Crl:NU-Foxn1 nu); n = 3 mice/group, error bars represent SD (representative of two independent experiments). i Maximum growth rates for tumors formed by CON or H2 HT1080 cells in h expressed as mm3/day; n = 3 mice/group, error bars represent SD (representative of two independent experiments)

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