Fig. 2

Receiver operating characteristic of CRISPOR using various off-target scores and versus the CRISPR Design website (http://crispr.mit.edu/). We used 26,034 putative off-targets identified by CRISPOR as the elements classified by the tools. The MIT website has a search depth of four mismatches, so off-targets with more than four mismatches were not considered for this graph. MIT score refers to the MIT off-target score as calculated by the CRISPOR website, MIT Website refers to the MIT off-target score as calculated by the CRISPR Design website (http://crispr.mit.edu/). For each scoring method, shown are the True positive rate (TPR)/False positive rate (FPR) when classifying 143 validated off-targets with a mismatch count of up to four, one of the PAMs NAG/NGA/NGG, and a minimum modification frequency of 0.1 %. The arrow marks the performance when using a CFD score cutoff value of 0.023. It leads to a 98 % true positive rate and a false positive rate of 43 %