Fig. 5From: ChromNet: Learning the human chromatin network from all ENCODE ChIP-seq data a Proximity ligation assay showing MYC and HCFC1 interaction in the nucleus. Representative micrographs show DAPI nuclear staining (left), proximity ligation signal (middle), and overlay (right) at 20× magnification, with insets at 100× magnification. Top: Cells probed with MYC antibody alone. Middle: Cells probed with HCFC1 antibody alone. Bottom: Cells probed with both antibodies. b Proximity ligation assay signal quantified as number of foci per nucleus, with 254, 293, and 381 nuclei quantified for the MYC antibody alone, the HCFC1 antibody alone, and both antibodies together, respectively. Individual values (gray dots) and mean ± standard deviation black bars from three biological replicates are shown; **** p<0.0001, one-way analysis of variance with Bonferroni post test. Quantifications for each independent replicate are shown in Additional file 1: Figure S20. PLA proximity ligation assayBack to article page