Fig. 4

More than 71 % of the transcripts that co-IP with the PfAlba1 complex directly bind to recombinant PfAlba1 in vitro. a The in vitro RNA pull-down protocol. GST or GST-PfAlba1 were immobilized on glutathione-agarose magnetic beads and incubated with total P. falciparum RNA prepared from a mixed trophozoite and schizont culture of 3D7 in the presence of protease and RNAse inhibitors. Bound RNAs were detected by strand-specific RNA-seq, with GST serving as a negative control. b Total RNA, or RNAs bound to GST or GST-PfAlba1 were resolved by denaturing polyacrylamide gel electrophoresis and visualized by Sybr Gold staining. Size marker = 0.1–2 kb RNA Ladder (Invitrogen, Life Technologies; nt = nucleotides). c A Gaussian density kernel estimate of the distribution of gene ranks according to mRNA abundance of the 1993 transcripts that bound to GST-PfAlba1. Refer to legend of Fig. 3c for details. d Fourier phase distribution of the normalized RNA-seq data of the 1993 GST-PfAlba1-bound transcripts. Refer to legend of Fig. 3d for details. e Venn diagrams were used to represent the overlap of transcripts identified in the co-IPed (co-IP; Additional file 2) and in vitro-bound (In vitro; Additional file 4) datasets