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Table 1 Method description

From: Errors in RNA-Seq quantification affect genes of relevance to human disease

Method

Aligner

Quantification

Quantification notes

star.htseq. u

STAR 2.4.0

htseq-count (HTSeq 0.6.1)

-m union

star.htseq.ine

STAR 2.4.0

htseq-count (HTSeq 0.6.1)

-m intersection-strict

star.htseq. is

STAR 2.4.0

htseq-count (HTSeq 0.6.1)

-m intersection-nonempty

tophat.htseq. u

TopHat 2.0.12

htseq-count (HTSeq 0.6.1)

-m union

tophat.htseq.ine

TopHat 2.0.12

htseq-count (HTSeq 0.6.1)

-m intersection-strict

tophat.htseq. is

TopHat 2.0.12

htseq-count (HTSeq 0.6.1)

-m intersection-nonempty

star.cufflinks

STAR 2.4.0

Cufflinks 2.2.1

 

star.cufflinks.mr

STAR 2.4.0

Cufflinks 2.2.1

--multi-read-correct

tophat.cufflinks

TopHat 2.0.12

Cufflinks 2.2.1

 

tophat.cufflinks.mr

TopHat 2.0.12

Cufflinks 2.2.1

--multi-read-correct

sailfish

NA

Sailfish 0.6.3

quant.sf

sailfish

NA

Sailfish 0.6.3

quant_bias_corrected.sf

  1. A description of the RNA-Seq alignment and quantification methods used. NA not applicable