Figure 3

Modulation of miR-221, miR-222 and miR-21 precursors in glioblastoma cell lines upon ADAR2 and ADAR2 E/A expression. (a) Pri-miR-221, pri-miR-222 and pri-miR-21 expression levels analyzed by qRT-PCR in U118 untreated (ctrl, dark gray), U118 over-expressing ADAR2 (ADAR2, medium gray) and U118 over-expressing ADAR2 E/A (ADAR2 E/A, light gray) cell lines. Each sample was normalized to GAPDH mRNA levels. Mean ± standard error of the mean (s.e.m.) (n = 3), **P < 0.01. (b) The same set of experiments in (a) was performed in the A172 cell line. (c) Pre-miR-21 expression levels in U118 (upper panel) and A172 (bottom panel) cell lines were analyzed by qRT-PCR. For the pre-miRs assay each sample was normalized to RNU6B levels. Mean ± s.e.m. (n = 3), **P < 0.01, *P < 0.05. The expression levels were calculated as a relative-fold increase compared with untreated cells (ctrl) arbitrarily set to 1.