Figure 1

Schematic diagram of a high-throughput screen of transcription factor overexpression constructs. We cloned 206 of 214 annotated DNA binding proteins (TFs) into a plasmid that placed the tagged protein under control of a tetracycline inducible promoter and fused the TF to a FLAG tag. Each of these TFs was then induced for one doubling period (approximately 18 h) and analyzed via expression profiling and ChIPseq [19]. Expression profiles were characterized using microarrays that covered both strands of the genome with a probe every approximately 100 bp.