Table 1 Summary of V H DJ H sequencing results
Pair number | Sample ID | Diagnosis/relapse | Number of unique VHDJHrearrangements | Number of unique VHDJHrearrangement per 103mapped reads | Dominant VHDJHrearrangement | Dominant rearrangement percentage | Number of sub-clones (>10X) within dominant VHDJH | Number of sub-clones (>10X) per 103dominant VHDJHalignments |
|---|---|---|---|---|---|---|---|---|
1 | 1D | Diagnosis | 311 | 0.69 | IGHV4-34 IGHD3-22 IGHJ5 | 96.9% | 1,591 | 3.60 |
1R1 | Relapse | 265 | 0.50 | IGHV4-34 IGHD3-22 IGHJ5 | 94.9% | 2,109 | 4.07 | |
1R2 | Relapse | NA | NA | NA | NA | NA | NA | |
1R3 | Relapse | 396 | 0.66 | IGHV4-34 IGHD3-22 IGHJ5 | 94.5% | 2,487 | 4.26 | |
2 | 2D | Diagnosis | 315 | 0.72 | IGHV4-59 IGHD6-19 IGHJ5 | 82.2% | 1,963 | 4.53 |
2R1 | Relapse | 3,131 | 7.27 | IGHV4-59 IGHD6-19 IGHJ5 | 88.9% | 1,372 | 3.44 | |
2R2 | Relapse | NA | NA | NA | NA | NA | NA | |
3 | 3D1 | Diagnosis | 950 | 1.84 | IGHV3-49 IGHD2-8 IGHJ4 | 28.3% | 421 | 2.81 |
IGHV3-49 IGHD3-22 IGHJ4 | 27.0% | 596 | 2.13 | |||||
3R2 | Relapse | 745 | 1.29 | IGHV3-49 IGHD3-22 IGHJ4 | 29.2% | 608 | 2.41 | |
IGHV3-49 IGHD2-8 IGHJ4 | 28.0% | 781 | 3.17 | |||||
4 | 4D | Diagnosis | 9,158 | 23.08 | IGHV3-7 IGHD4-17 IGHJ6 | 69.7% | 1,318 | 4.75 |
4PR | Progression | 1,785 | 4.34 | IGHV3-7 IGHD4-17 IGHJ6 | 43.0% | 933 | 5.23 | |
5 | 9D | Diagnosis | 2,911 | 4.27 | IGHV4-34 IGHD6-13 IGHJ6 | 47.5% | 1,381 | 4.16 |
IGHV1-18 IGHD6-13 IGHJ2 | 39.6% | 1,075 | 3.96 | |||||
9R | Relapse | 18,683 | 25.72 | IGHV1-18 IGHD6-13 IGHJ2 | 9.2% | 328 | 4.57 | |
IGHV4-34 IGHD6-13 IGHJ6 | 3.5% | 154 | 5.33 | |||||
6 | 12D | Diagnosis | 7,570 | 22.12 | IGHV3-74 IGHD4-17 IGHJ4 | 25.2% | 0 | 0 |
12R | Relapse | 7,299 | 13.78 | IGHV3-23 IGHD6-13 IGHJ4 | 24.1% | 496 | 3.86 | |
7 | 13D1 | Diagnosis | 7,931 | 27.09 | IGHV3-23 IGHD3-9 IGHJ6 | 82.4% | 870 | 5.14 |
13D2 | Diagnosis | 2,436 | 6.65 | IGHV3-23 IGHD3-9 IGHJ6 | 89.6% | 718 | 5.74 | |
13R | Relapse | 1,204 | 3.71 | IGHV3-23 IGHD3-9 IGHJ6 | 93.1% | 858 | 5.27 | |
8 | 14D | Diagnosis | 4,172 | 12.07 | IGHV1-18 IGHD6-13 IGHJ2 | 48.0% | 819 | 4.89 |
IGHV4-34 IGHD6-13 IGHJ6 | 24.1% | 327 | 3.76 | |||||
14R | Relapse | 1,206 | 3.84 | IGHV1-18 IGHD6-13 IGHJ2 | 57.7% | 883 | 4.81 | |
IGHV4-34 IGHD6-13 IGHJ6 | 32.1% | 421 | 3.96 | |||||
9 | 15D1 | Diagnosis | 8,727 | 15.47 | IGHV1-18 IGHD6-13 IGHJ2 | 23.7% | 471 | 4.97 |
IGHV3-48 IGHD2-2 IGHJ4 | 26.8% | 0 | 0 | |||||
15D2 | Diagnosis | 955 | 2.83 | IGHV1-18 IGHD6-13 IGHJ2 | 37.3% | 18 | 2.73 | |
IGHV4-34 IGHD6-13 IGHJ6 | 35.4% | 0 | 0 | |||||
15R | Relapse | 1,296 | 4.62 | IGHV3-48 IGHD2-2 IGHJ4 | 37.1% | 530 | 4.95 | |
IGHV1-18 IGHD6-13 IGHJ2 | 33.5% | 471 | 5.00 | |||||
10 | 16D | Diagnosis | 11,303 | 12.10 | IGHV1-18 IGHD6-13 IGHJ2 | 55.2% | 867 | 1.61 |
16R | Relapse | 1,875 | 2.67 | IGHV1-18 IGHD6-13 IGHJ2 | 44.8% | 1,079 | 3.42 | |
11 | F6D | Diagnosis | 786 | 1.04 | IGHV3-23 IGHD3-22 IGHJ4 | 72.5% | 991 | 4.22 |
F6PR | Progression | 2,135 | 4.03 | IGHV3-23 IGHD3-22 IGHJ4 | 61.0% | 1,245 | 5.29 | |
12 | F7D | Diagnosis | 3,491 | 6.07 | IGHV4-34 IGHD3-22 IGHJ6 | 45.2% | 231 | 0.61 |
F7R | Relapse | 6,115 | 14.58 | IGHV4-34 IGHD3-22 IGHJ6 | 40.0% | 82 | 0.33 | |
13 | SPF6-1 | Diagnosis | 1,160 | 2.41 | IGHV3-7 IGHD3-10 IGHJ4 | 90.0% | 1,691 | 3.83 |
SPF6-2 | Diagnosis | 828 | 1.75 | IGHV3-7 IGHD3-10 IGHJ4 | 93.1% | 1,854 | 3.93 | |
SPF6-3 | Relapse | 719 | 1.54 | IGHV3-7 IGHD3-10 IGHJ4 | 95.8% | 1,754 | 3.85 | |
14 | SPF10-1 | Diagnosis | 1,463 | 3.17 | IGHV3-49 IGHD3-10 IGHJ5 | 67.8% | 1,425 | 3.65 |
SPF10-2 | Relapse | 1,731 | 3.73 | IGHV3-49 IGHD3-10 IGHJ5 | 30.2% | 777 | 4.37 |