Figure 9

WDR55 is a target of SVP in reproductive tissues. (a) Expression analyses of WDR55, RNA was extracted from wild type Col-0 and svp-41 agl24-2 ap1-12 triple mutant inflorescences. (b) ChIP-PCR validation of WDR55 region bound by SVP in reproductive tissues; (c-j) phenotypic analysis of (c) wild-type and (d-j) wdr55-2 flowers: (d) flower of wdr55-2 mutant showing reduced size of sepals and petals; (e) flower of wdr55-2 mutant with unfused sepals, (f) arrow indicates unfused carpel, (g) arrow indicates chimeric sepal bearing stigmatic tissue on the top, (h) asymmetric development of wdr55-2 flower, (i) wdr55-2 flowers often develop new flowers in whorls 1 and 2; (k-n) in-situ hybridization of (k) wild-type, and (l-n) wdr55-2 using AG specific probe: (k) in wild-type inflorescence AG is expressed from late stage 2 and its expression is restricted to the third and fourth whorl (im is inflorescence meristem), (l) ectopic expression of AG in staminoid petal of wdr55-2 flower, (m) carpelloid sepals expressing AG in wdr55-2 flower, (n) early stages of flower development in wdr55-2 mutant in which the expression of AG is already detectable. The scale bar represents 20 μm.