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Figure 4 | Genome Biology

Figure 4

From: High-throughput RNA interference screening using pooled shRNA libraries and next generation sequencing

Figure 4

Quantitative PCR quantification of PCR products. Quantitative PCR (qPCR) assay designed to detect and quantify all amplifiable solexa molecules (using oligos p5/p7 and SybrGreen) or shRNA-specific PCR products (using Taqman, amplification primers p5/p7 and a dual-labeled probe). (a) shRNA PCR products quantified against a library of known concentration. (b) Standard curve constructed using a ten-fold dilution series covering 100, 10, 1 and 0.1 pM. (c) Agilent electrophoresis profile of reference library.

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Genome Biology

ISSN: 1474-760X

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