Advertisement
Table 1 Primers sequences for GeNorm
| Symbol | Name | NCBI accession number | Localization | Span intro | Sequences |
|---|---|---|---|---|---|
| ACTB | Beta actin | NC_001101 | Chromosome 7 | No | Fw:5'-AGTGTGACGTGGACATCCGCA-3' |
| Rev:5'-GCCAGGGCAGTGATCTCCTTCT-3' | |||||
| HPRT1 | Hypoxanthine purine synthesis phosphoribosyl-transferase 1 | NC_000023.9 | Chromosome x | Yes | Fw:5'-ATGACCAGTCAACAGGGGACAT-3' |
| Rev: 5'-CAACACTTCGTGGGGTCCTTTTCA-3' | |||||
| YWHAZ | Tyrosine 3-monooxygenase/S tryptophan 5-monooxygenase activation protein, zeta polypeptide | NC_000008.9 | Chromosome 8 | Yes | Fw: 5'-CGTTACTTGGCTGAGGTTGCC-3' |
| Rev: 5'-GTATGCTTGTTGTGACTGATCGAC-3' | |||||
| GNB2L1 | Guanine nucleotide binding protein, beta polypeptide 2-like 1 | NC_000005.8 | Chromosome 5 | Yes | Fw: 5'-GAGTGTGGCCTTCTCCTCTG-3' |
| Rev: 5'-GCTTGCAGTTAGCCAGGTTCC-3' | |||||
| B2M | Beta-2-microglobulin | NC_000015.8 | Chromosome 15 | No | Fw: 5'-GGAGAGAGAATTGAAAAAGTGGAGC-3' |
| Rev: 5'-GGCTGTGACAAAGTCACATGGTT-3' | |||||
| GAPDH | Glyceraldehyde-3-oxidoreductase phosphate dehydrogenase | NC_000012.10 | Chromosome 12 | No | Fw: 5'-AGCTGAACGGGAAGCTCACT-3' |
| Rev: 5'-AGGTCCACCACTGACACGTTG-3' |
