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Figure 6 | Genome Biology

Figure 6

From: Expressed Alu repeats as a novel, reliable tool for normalization of real-time quantitative RT-PCR data

Figure 6

Normalization to EAR and GeNorm to evaluate TNNC1 mRNA levels, a gene specifically expressed in human skeletal muscle. (a) Application of the GeNorm algorithm to six genes to identify the three most stable housekeeping genes in human skeletal muscle. Average expression stability values (M) of remaining control genes during stepwise exclusion of the least stable control gene in the human skeletal muscle of four control participants. (b) Median skeletal muscle TNNC1 mRNA levels versus EAR and versus the three most stable control genes in four control participants. Samples from the National Neurological Institute-IRCCS "Carlo Besta", Milan (Italy). Pearson's correlation r = 0.94; P < 0.05

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Genome Biology

ISSN: 1474-760X

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