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Table 2 Comparison of microarray and RT-qPCR results for housekeeping or stable genes

From: Global expression analysis of the brown alga Ectocarpus siliculosus (Phaeophyceae) reveals large-scale reprogramming of the transcriptome in response to abiotic stress

ID

Genome ID

Name

Maximum change ARRAY

Maximum change QPCR

Function

LQ0AAB30YA12FM1.SCF

[Esi0298_0008]

Dyn

0.23

0.77

Dynein

CL1914Contig1

[Esi0021_0024]

ARP2.1

0.22

0.44

Actin related protein

CL3Contig2

[Esi0387_0021]

EF1A

0.08

0.46

Elongation factor 1 alpha

CL8Contig12

[Esi0053_0059]

TUA

0.57

0.91

Alpha tubulin

CL1073Contig1

[Esi0054_0059]

UBCE

0.22

0.38

Ubiquitin-conjugating enzyme

CL29Contig4

[Esi0302_0019]

UBQ

0.18

0.82

Ubiquitin

CL461Contig1

[Esi0072_0068]

R26S

0.22

n/a

Ribosomal protein S26

  1. The table displays the maximum log2-ratio between any stress and the control condition for both the microarray and the RT-qPCR analysis. No RT-qPCR value is available for R26S, as this gene was used for normalization of the RT-qPCR samples. ID corresponds to the name of the sequence on the array.