Figure 6

Immunohistochemical analysis of epidermal proteins encoded by array-identified estrogen-regulated genes demonstrates altered expression in wounds from both old humans and ovx young female mice. (a-d) Representative immunohistochemical localization of the epidermal differentiation markers loricrin and involucrin demonstrates decreased expression in wound epidermis from old males compared to wounds from young males. (e-l) Representative immunohistochemical localization of the epidermal proteins keratin 16, loricrin, involucrin and desmocollin 1, which are decreased in wound epidermis from young ovx mice compared to wounds from mice with intact ovaries. The scale bar in (l) represents 70 μm (a-d), 300 μm (e-f), and 140 μm (g-j).