Figure 1

Nuclear run-on analysis shows that actD halts transcription in Plasmodium falciparum. Actinomycin D (actD) was added to synchronous cultures in the ring and late schizont stages. Time points were then taken before addition of actD and then at 0, 7.5, and 15 min intervals after addition of drug. The samples were normalized such that the no actD sample was normalized to 100% transcription.