Figure 4From: Genome-wide identification of functionally distinct subsets of cellular mRNAs associated with two nucleocytoplasmic-shuttling mammalian splicing factorsU2AF65 associates with fully spliced mRNAs. Agarose gel electrophoresis of RT-PCR reactions designed to check the splicing status of mRNAs present in control (Mock) and anti-U2AF65 (U2AF) RNA-immunoprecipitations. For each target, the transcript exon/intron structure is represented to scale and primer localization and expected size of fully spliced products are indicated. IP, immunoprecipitation; RT-PCR, reverse transcription polymerase chain reaction; U2AF, U2 small nuclear RNP auxiliary factor.Back to article page