Figure 2

Expression patterns of Arabidopsis genes encoding 7TMpR candidates and G-protein subunits among tissues. The figure was modified from an output of the Meta-Analyzer of Genevestigator (last updated in November 2005), which illustrates expression levels of each gene in different organs. Relative expression levels of a gene in different organs/tissues are given as heat maps in blue-scale coding that reflects absolute signal values, where darker colors represent stronger expression. All gene-level profiles are normalized for coloring such that, for each gene, the highest signal intensity obtains a value 100% (shown in the darkest blue and marked with an asterisk) and absence of signal obtains a value 0% (shown in white). All GeneChip data was processed using Affymetrix MAS5.0. Special precaution is required for gene expression in certain cell types (for example, pollen), since difference in normalization may achieve different results. Probe-sets of five 7TMpR candidates (At1g15620. At1g75000, At4g21570, At4g36850, and At5g23990) were not present in the 22K chip, and, therefore, their tissue-specific expression could not be assessed. For At2g35710, two probe-sets (265797_at^a and 265841_at^b) were designed on the chip. Gene names for those belonging to the MtN3 family are shown in boldface and marked with an asterisk. Genes encoding G-protein subunits (AGB1, GPA1, AGG1, and AGG2) as well as two reported 7TMpRs (RGS1 and GCR1) are labeled accordingly in boldface.