Figure 2

Diagrammatic representation of the evolutionary history of chromosome 13. (a) Marker order arrangement in the studied species, from which the arrangement of the mammalian ancestor (MA) and primate ancestor (PA) was derived (see text). N in a red circle stands for new centromere. The number that identifies the chromosome in each species is reported on top of the chromosome. The black letters on the left of each primate chromosome refer to the panel of BAC probes reported in Table 1 (human BACs); letters on cattle (BTA), pig (SUS), horse (ECA), and cat (FCA) chromosomes refer to BACs reported in Additional data file 1, obtained by library screening or from published databases (see text). Letters in red are the additional probes used to delimit chromosomal breakpoints or featuring unusual results (see N and H8 in the cat). Letter with asterisk indicate BACs identified on the radiation hybrids mapping data and used to fill gaps due to library screenings failure (see Table 1 and text). The long arm of cat chromosome A1 was shortened because of space constraint. The red lightning indicates chromosome break. (b) Results of FISH experiments of the H1 to H9 clones (Table 1) on OWM species (left) and in pig (SUS, right). Clones in red are duplicated. In OWM, the clones not reported in the figure failed to yield FISH signals. For details see text. (HOM = Hominoidea;HSA-GA = Homo Sapiens-Great Apes Ancestor).