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Table 4 Summary statistics of DNA polymorphisms analyzed in the study

From: Ancient origin and evolution of the Indian wolf: evidence from mitochondrial DNA typing of wolves from Trans-Himalayan region and Pennisular India

 

Mitochondrial domain used for taxonomic comparisions

Sequence Parameters

Complete Cyto-B gene

Partial Cyto-B gene

16S rRNA gene

D-loop (CR-I) 233 bp

D-loop (CR-I) 545 bp

D-loop (CR-I) 651 bp

D-loop (CR-I) 675 bp

D-loop Region-II

Sequences compared

5

35

27

80

50

33

24

11

Sequence size compared (bp)

1140

332

554

233

545

651

675

294

Sequence size without gaps/'N' (bp)

1132

280

554

210

505

608

656

252

Polymorphic nucleotides

232

77

32

35

72

73

75

22

Singleton polymorphic nucleotides

178

34

.

14

33

39

44

16

Informative nucleotides

54

43

32

21

39

34

31

6

Base composition (average)

        

A :

28.9%

28.2%

26.7%

30.5%

26.9%

27.3%

27.2%

33.6%

C :

27.6%

23.0%

20.3%

24.0%

27.1%

26.5%

25.6%

21.4%

G :

14.2%

16.6%

21.7%

12.3%

14.9%

15.3%

16.3%

11.3%

T :

29.4%

32.2%

31.3%

33.2%

31.1%

30.9%

30.9%

33.7%

Observed Transition/transversion ratios (over all sites/sequences/pair-wise comparisions)

Average

4.15

8.70

11.65

19.57

9.89

7.60

6.96

14.20

Mimimum

2.80

0.00

5.70

0.00

0.00

0.00

0.00

0.00

Maximum

18.75

26.00

11.00

15.00

23.00

24.00

25.00

12.00

Observed substitution statistics

   Over all sites/sequences/pair-wise comparisions

   No. of haplotypes

5

18

6

36

39

28

 

10

   Number of Average substitutions

106.0+66.0

12.35+9.57

7.21+0.93

4.97+0.15

9.65+0.83

11.15+1.7

 

5.53+1.5

   Nucleotide diversity (%)

9.39+4.87

4.37+1.15

1.30+0.50

2.37+0.04

1.91+0.04

1.83+0.03

 

2.19+0.03

   Ks (%)

57.2+48.6

19.2+16.5

      

   Ka (%)

1.49+0.79

0.77+0.80

      

   HW vs. GW and wolf/dog popun from elsewhere

   Number of Average substitutions

   

14.1+1.6

20.7+1.9

21.3+1.9

 

7.1+1.6

   Miminum-maximum substitutions

   

11-16

17-24

18-25

 

5-9

   Median (Mode) substitutions

   

14 (16)

20 (19)

21 (21)

 

7 (7)

   GW vs. HW and wolf/dog popun from elsewhere

   Number of Average substitutions

   

13.4+1.3

17.3+1.6

18.0+2.0

 

5.1+1.6

   Miminum-maximum substitutions

   

10-17

14-21

14-21

 

4-9

   Median (Mode) substitutions

   

13 (13)

17 (17)

18 (19)

 

5 (4)

   Between different wolf/dog popun excluding HW/GW from India

   Number of Average substitutions

   

4.5+2.23

7.4+3.2

7.6+3.4

 

3.1+1.3

   Miminum-maximum substitutions

   

0-10

0-15

0-15

 

0-5

   Median (Mode) substitutions

   

4 (4)

8 (7)

8 (7)

 

3 (3)