Adult midgut expressed sequence tags from the tsetse fly Glossina morsitans morsitansand expression analysis of putative immune response genes

Table 1 Serine protease-related group of putative immune-related genes chosen for arraying

Contig/EST	Length (bp)	E value	Homology	Putative function
Gmm-1598 *	850	3e-41	CG3066 (dm)	Serine carboxypeptidase
Tse112d02.q *	473	4e-63	Easter (dm)	Serine endopeptidase
Tse91h03 *	576	1e-56	CG3066 (dm)	Serine carboxypeptidase
Tse129g02 *	476	3e-46	PPAF (tm)	PPO activation
Tse105c10.q *	557	4e-41	TepIV (dm)	Complement c3/α2 macroglobulin
Tse48e12.q *	553	7e-38	TepIV (dm)	Complement c3/α2 macroglobulin
Gmm-3334 *	1,454	1e-102	Sp6 (dm)	Serpin
Gmm-3352 *	2,529	1e-109	Sp5 (dm)	Serpin
Gmm-2356 *	962	4e-44	CG9460 (dm)	Serpin
Gmm-2594 *	570	2e-38	CG9455 (dm)	Serpin
Gmm-0033 *	452	1e-21	Sp6 (dm)	Serpin
Tse72e04.q *	436	5e-37	Spn43Ab (dm)	Serpin
Gmm-2766	450	7e-06	AAK57342.1 (ti)	Serpin
Tse72e08 *	543	2e-23	CG6680 (dm)	Serpin
Tse94c04.q *	560	2e-52	CG5392 (dm)	Serpin

The contig (Gmm-) or clone identifier (Tse) is given for each gene. A 'q' following the clone identifier indicates sequencing was from the 39 end of the clone. The tables include the lowest BLASX E value (most significant similarity) together with putative function based on this homology. dm, Drosophila melanogaster; cb, Chrysomya bezziana; sp, Sarcophaga peregrina; ds, Drosophila simulans; tm, Tenebrio molitor; ti, Triatoma infestans; bm, Bombyx mori; ce, Caenorhabditis elegans; ag, Anopheles gambiae; gm, G. m. morsitans. *All genes with an Anopheles gambiae homolog at 1e-08 or less.

ISSN: 1474-760X