Figure 2

Effect of UV cross-linking intensity on retention of unmodified rat 70-mer oligonucleotides. The 70-mer oligonucleotides (50 μM) were printed onto TeleChem SuperAmine slides that were subjected to different UV cross-linking intensities and hybridized to Cy3- and Cy5-labeled targets derived from reverse transcribed rat heart and brain total RNA. A representative array section is shown for each UV cross-linking energy. (a,d) Experiments in which brain and heart targets were labeled with Cy3 and Cy5, respectively. (b,c) Flip-dye experiments in which brain and heart targets were labeled with Cy5 and Cy3, respectively. Each panel is representative of three to four independent experiments.