Figure 3

Splicing, mouse-human conservation patterns, and tissue origin of sense and antisense ESTs from UniGene cluster Hs.288835. (a) The graph depicts the exon-intron splicing structures of transcript sequences belonging to UniGene cluster Hs.288835. Organization of the figure as for Figure 2. The BOU mRNA (GenBank accession NM_014430) is oriented from left to right with respect to genomic contig Hs14_19739_24 of the NCBI human genome assembly. The mRNA encodes CIDEB (cell-death inducing DFFA-like effector B). With no exceptions, the sense-oriented ESTs have splicing patterns that are consistent with that of the mRNA. The antisense ESTs, however, consistently overlap with intronic sequence of the sense transcript, suggesting that they are derived from a distinct RNA species (presumably unspliced, at least in the region that we are observing). (b) A plot of EST numbers in the CIDEB cluster against orientation. The y-axis indicates the number of sense or antisense ESTs observed in the CIDEB cluster, and the relative proportions arising from neoplastic versus non-neoplastic tissues are indicated. A significantly greater fraction of the antisense ESTs (34/46 = ~0.74) than the sense ESTs (3/15 = ~0.2) were derived from neoplastic tissues (p = ~0.0002 by chi-squared statistic).