Skip to main content
Fig. 5 | Genome Biology

Fig. 5

From: SeATAC: a tool for exploring the chromatin landscape and the role of pioneer factors

Fig. 5

SeATAC detects Etv2 binding sites with increased chromatin accessibility during Etv2-induced EB differentiation and MEF reprogramming. a, b The Venn diagrams show the number of Etv2 motifs with increased chromatin accessibility identified by SeATAC, MACS2, and NucleoATAC, in a Etv2-induced MEF reprogramming (undifferentiated MEFs vs. Flk1+ cells at 7 days post-induction) and b Etv2-induced EB differentiation (D2.5 EB vs. Flk1+ cells at 12 h post-induction). c The aggregated V-plot includes 1626, 222, and 2305 Etv2 motifs with increased chromatin accessibility identified by SeATAC only, MACS2 only and NucleoATAC only in ATAC-seq data of Etv2-induced EB differentiation (day 2.5 EB vs. Flk1+ cells at 12 h post-induction). Both raw V-plots and estimated V-plots are shown. The heatmap color indicates the normalized read density for raw counts (top) and the estimated read density for estimated read counts (bottom). d The barplots show the Gene Ontology (GO) terms that are significantly associated with the genes where the promoters (− 5000 to + 1000 bp region flanking the TSS) have Etv2 motifs with increased chromatin accessibility, identified by SeATAC, MACS2, and NucleoATAC. The y-axis showed the adjusted p-value of the pathway analysis

Back to article page