Skip to main content
Fig. 3 | Genome Biology

Fig. 3

From: Cell-specific clock-controlled gene expression program regulates rhythmic fiber cell growth in cotton

Fig. 3

The small peptide GhRALF1s inhibits fiber cell growth. a UMAP projection showing the fiber cell-specific expression of GhRALFs. b The sequence alignment of matured GhRALFs. Conserved amino acids are labeled in green color, and specific amino acids of GhRALF1 and GhRALF2 are labeled in blue and red color respectively. c The fiber growth phenotypes of in vitro cultured ovules treated with GhRALF1 and GhRALF2. Scale bar, 5 mm. d The H + -ATPase activity test for 1 DPA ovules with GhRALF treatment measured by Seahorse XF24 Extracellular Flux analyzer, the x- and y-axis denote extracellular acidification rate (ECAR) and relative time to small peptide injection. Distilled water (pH 7.0) was injected as a control. The dashed line indicates the time point of peptide treatment. e Volcano plot for the GhRALF1 stimulated DEGs (FDR ≤ 5% and fold change ≥ 2). See also Additional file 5: Table S4. f The action mode for the inhibition role of GhRALF1 on fiber cells. The secreted peptide might be recognized by the receptor on PM to transduce inhibitory signals to proton pump and auxin signaling

Back to article page