Fig. 7

Rapid methylation loss is driven by accessibility and transcription factor binding. A Genome browser snapshot depicting a recalcitrant DMR on the HOXD11 promoter. B Methylation levels in DMRs with a trend of 5mC loss (mCG on DoxWD-7days < 10%) or 5mC retain (mCG on DoxWD-7days > 10%). C Number and overlap of DMRs classified per methylation retention trend. D TF binding motif enrichments in DMRs, highlighted in bold, are those motifs confirmed by differential ChIP-seq signal. Average ENCODE MCF-7 TF ChIP-seq signal on E distal regulatory elements and F promoters. G ZF-D3A-wt ChIP-seq signal (background subtracted), H ATAC-seq signal, and I H3K27me3 levels (CPM) upon Dox induction at promoters and distal regulatory elements classified as per methylation retention trend. Dark blue lines depict DMRs that lose methylation and green lines depict DMRs that retain methylation