Fig. 2

The ZF-D3A can bind closed chromatin yet 5mC deposition is limited by nucleosome positioning. A Top motif in ZF-D3A-wt ChIP-seq peaks. B Overlap between ZF-D3A-wt peaks, ZF-D3A-peaks harboring the top motif displayed in A (75.9% of all peaks), and genomic regions exactly matching the CCCTCCTCCCCC sequence in the human genome (hg19 build). C Percentage of ZF-D3A-wt peaks in open chromatin, defined as noDox ATAC peaks. D Mean distribution of CG methylation, CH methylation (H = A, T, C), raw ATAC-seq, and nucleoATAC signal on ZF-D3A peaks. ZF-D3A peaks are classified as per overlap with ATAC-seq peaks, and centered on the ZF top motif. Dashed lines indicate the periodic peaks corresponding to nucleosome positions defined by the nucleoATAC signal in the Dox state. noDox and Dox correspond to ZF-D3A-wt, whereas Dox-mut corresponds to ZF-D3A-mut