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Fig. 3 | Genome Biology

Fig. 3

From: Using synthetic chromosome controls to evaluate the sequencing of difficult regions within the human genome

Fig. 3

Sequencing performance at microsatellites. a Read alignment, for HiSeq X Ten/PCR-free and ONT PromethION, at the synthetic unstable homopolymer BAT-25 microsatellite. The histogram shows the depth of coverage at each position, with aligned reads shown below. Any deletions in the aligned reads are indicated by black segments. b Frequency (log2) of deletions and insertions at small (≤ 5 nt), medium (> 5 nt and ≤ 15 nt), and large (> 15 nt) homopolymers. c Relative frequency of reads containing exact repeat size matches for the microsatellites in the Bethesda panel. a–c The colors represent the different sequencing technologies/preparation methods used to generate each of the seven sequenced libraries, such as HiSeq X Ten/PCR-free (purple), HiSeq 2500/PCR-based (red), NextSeq 500/PCR-based (blue), MGISEQ-2000 (yellow), and ONT PromethION (green)

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