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Fig. 6 | Genome Biology

Fig. 6

From: Landscape of transcription termination in Arabidopsis revealed by single-molecule nascent RNA sequencing

Fig. 6

Cleaved readthrough transcripts can transcribe into downstream genes and produce spliced and polyadenylated mRNAs in atxrn3. a Examples of genes with cleaved readthrough reads extended to downstream genes. Upper panel, wildtype; lower panel, atxrn3 mutant. The black dashed line indicates the poly(A) site. The red dashed box showed the magnified view of cleaved and polyadenylated reads at the subsequent poly(A) site. b Zoomed-in view of poly(A) reads aligned to gene AT1G73530 in two biological replicates of the wildtype and atxrn3 FLEP-seq libraries. c Read coverage of RNA-seq data [14] around the AT1G73540-AT1G73510 region in wildtype and atxrn3 mutant (log2 scale). d Model of AtXRN3-mediated transcription termination

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