Fig. 4

Downstream tRNA promotes efficient termination of upstream protein-coding genes. a, d Examples of Pol II genes that have tRNA gene immediately downstream, arranged in tandem (a) or convergent (d). The number of reads (n) is shown. The poly(A) transcript reads are shown in purple, and their poly(A) tail is shown in red. The readthrough transcript reads are shown in blue. The distance between the termination window end and tRNA is marked by bracket. b, e The distribution for the distance between each pair of the termination window end (TWE) and its downstream tRNA gene. Tandem orientation (b). Convergent orientation (e). c, f Read coverage of genes that have tRNA or non-tRNA genes immediately downstream. In the case of tandem orientation, the start of the downstream gene is used as a reference (c). In the case of convergent orientation, the end of the downstream gene is used as a reference (f). g Model for tRNA-dependent termination in Arabidopsis