Fig. 3

Differentiation trajectory of xylem cells. A The successive differentiation trajectory from xylem mother cells to mature xylem cells. Each dot indicates a single cell. The black arrow indicates the start of the trajectory. B Expression of representative marker genes (ACL5, PtrHB4, PtrHB7, and PtrHB8) at the beginning of the trajectory. The color bar indicates relative expression levels. C Heatmap showing expression levels of branch-dependent genes over pseudo-time. Representative marker genes are shown on the right of the heatmap. Both sides of the heatmap are the end of pseudo-time. D Bar chart showing results of GO enrichment analysis of the identified cell clusters. Expression of ABR1 (E) and ESK1a (F) at the trajectory and results of RNA in situ hybridization of them with the sense probe as a negative control. Scale bars, 100 μm. G Representative marker genes (PdDUF579-9, PtAP66, PtAP17, PtrMAN6, XCP1, and VND1) expressed in XSCW branch. The color bar indicates relative expression levels