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Fig. 2 | Genome Biology

Fig. 2

From: Detection of SARS-CoV-2 RNA using RT-LAMP and molecular beacons

Fig. 2

Reaction progression curves comparing RT-LAMP using the Penn primer set assayed using an intercalating dye and the Penn_LFMB_S1 molecular beacon in the same reactions. a Conventional RT-LAMP assay using non-specific dye to detect amplification of synthetic SARS-CoV-2 RNA diluted in water. Time after reaction initiation (x-axis) is compared to the relative fluorescence intensity (y-axis). The copy numbers of SARS-CoV-2 RNA in the reaction mixtures are shown in the key at the bottom. b Detection of the amplification of SARS-CoV-2 RNA using a LAMP-BEAC molecular beacon in the same reactions shown in A. Lines are colored as in A. c, d Thermal melting curves to characterize amplification products. The results shown are for the same reactions as in a and b. Reaction products were cooled to room temperature, then slowly heated for the melt curve analysis. c Characterization of the fluorescence intensity produced by non-specific intercalating dye (y-axis) with RT-LAMP end products over varying temperatures (x-axis). Lines are colored as in a. d Characterization of the fluorescence intensity produced by a LAMP-BEAC molecular beacon with RT-LAMP end products over varying temperatures. Markings as in c

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