Fig. 2

Differential methylation reveals a subset of hippocampus samples originate from the dentate gyrus. a Principal component analysis of distances derived from average autosomal CpG methylation in 1-kb bins. Data shown are from this study and from [11] as indicated. b Hierarchical clustering of hippocampus samples based on the average methylation per sample in the CG-DMRs identified between the two hippocampus groups. c Hierarchical clustering of hippocampus samples based on the average methylation per sample of hippocampal CG-DMRs overlapping dentate gyrus marker genes. The primary marker of the dentate gyrus, PROX1, is boxed. d Example hippocampal CG-DMRs in the PROX1 gene with average methylation values calculated from NeuN+ nuclei isolated from indicated tissue groups and regions of differential methylation shaded pink