Skip to main content
Fig. 2 | Genome Biology

Fig. 2

From: Deep sequencing of pre-translational mRNPs reveals hidden flux through evolutionarily conserved alternative splicing nonsense-mediated decay pathways

Fig. 2

a Distribution of the number of exon junctions in all annotated protein-coding (gray) or NMD (blue) transcripts. b Distribution of protein-coding (top) and NMD (bottom) transcripts per million (TPM) in each library type (colors as in Fig. 1a), binned based on indicated number of exon junctions per transcript. Numbers in bottom table: total number of expressed isoforms per exon junction number bin. c Scatterplots comparing TPMs between EJC RIPiT-Seq and RNA-Seq (+) libraries for protein-coding (left) and NMD (right) isoforms. Transcripts from Fig. 1 are noted. N: number of detected transcripts (out of all annotated transcripts of that type). Dashed black line: x = y. d Distribution of read counts at unique junctions, binned based on transcript biotypes [3]. Numbers below: Total number of unique junctions detected in our libraries per transcript biotype. e Ratios of read counts at unique junctions between two indicated library types, binned based on transcript biotype [3]. For b, d, e, results of one-way ANOVA and Tukey’s post hoc significance tests comparing EJC RIPiT-Seq to RNA-Seq libraries are indicated as *P < 0.05, **P < 0.01, ***P < 0.005, ****P < 0.0001

Back to article page