Fig. 1

The Tug1 lncRNA locus is highly conserved and ubiquitously expressed. a Tug1 mouse genomic locus (shown inverted). UCSC Genome Browser tracks for RNA sequencing (RNA-seq), RNA polymerase II (Pol II), histone 3 lysine 4-trimethylation (H3K4me3), H3K36me3, and H3K4me1 occupancy in the testis and H3K9me3 occupancy in the brain are depicted. PhyloCSF scores across the locus are shown. Chromosomal coordinates of the mouse Tug1 gene are indicated (mm9). b Upper panel: schematic of the nucleotide conservation alignment for mouse and human Tug1/TUG1. Red lines indicate conserved nucleotides. Chromosomal coordinates of the Tug1/TUG1 gene for both species are indicated. Lower panel: distribution of sequence identity for orthologous divergent and intergenic lncRNAs between mice and humans. The x-axis shows increasing conservation rank. Tug1 and other well-characterized lncRNAs are highlighted. c RNA-seq expression levels of Tug1 in a panel of mouse and human tissues. d RNA in situ hybridization of Tug1 RNA in a mouse embryo at embryonic day 10.5 (E10.5). e Maximum intensity projections of Tug1 single-molecule RNA FISH (gray) on murine 3T3 and human BJ fibroblasts. The nucleus is stained with DAPI (blue). Scale bar is 5 μm