Fig. 5

ADAR1- and ADAR2-specific sites are comparable. a Analysis of evolutionarily conserved A-to-I editing events across the genotypes. Average editing for each site was calculated plotted with reference to the levels at each site identified by Pinto et al. [29]. ADAR1/ADAR2 shared sites are defined as having > 10% and < 150% editing compared to the average editing rate of the WT and Adar1^E861A/+Adarb1^+/− (dHet) samples combined (WT+dHet); ADAR1-specific sites have < 10% editing of this site in the Adar1^E861A/E861A samples and unchanged editing in the Adarb1^−/− compared to WT+dHet; ADAR1-specific/ADAR2 inhibits sites have < 10% editing of this site in the Adar1^E861A/E861A samples and > 150% editing of WT+dHet levels in the Adarb1^−/−; ADAR2-specific sites have < 10% editing of this site in the Adarb1^−/− samples and unchanged editing in the Adar1^E861A/E861A compared to WT+dHet; ADAR2-specific/ADAR1 inhibits sites have < 10% editing of this site in the Adarb1^−/− samples and > 150% editing of WT+dHet levels in the Adar1^E861A/E861A. b Quantitation of the numbers of sites (≥ 50 read coverage and an editing rate of ≥ 0.01 (≥ 1%)) and genomic location across genotypes. ADAR1- or ADAR2-specific sites were defined as having < 10% editing of a site in the one genotype and retained editing in the alternative genotype. The percentage of sites that are ADAR1 or ADAR2 specific is indicated in brackets as a percent of the total number of sites for each location. The sequence context of the editing sites for each classification was derived with Seqlogo. The distribution of editing in B1 and B2 SINEs was mapped from the total sites identified in each genotype. c The genomic distribution/repeat type and average editing level for the ADAR1 and ADAR2 sites compared to the all sites observed in the control (WT+dHet genotype combined). Box and whiskers plot with 5–95 percentile shown. No significant difference between genotypes or P value as indicated (ANOVA with multiple comparisons correction)