Fig. 5
From: Characterizing the interplay between gene nucleotide composition bias and splicing
Nucleotide composition bias, gene features, and transcription. a Correlation between the GC content of splicing factor–activated exons and the GC content of their hosting genes; r = Pearson correlation coefficient. b Box plots representing the GC content (%, left panel), the intron size (middle panel), and the size (right panel) of genes hosting GC exons or AT exons. The red lines indicate the median values calculated for control exons. “$” and “*” correspond to Student’s test (left panel and right panel) and Wilcoxon’s test (middle panel) P value < 2 × 10−4 when comparing genes hosting GC exons and AT exons and when comparing genes hosting GC exons or AT exons to control genes, respectively. c Density of reads obtained after immunoprecipitation of RNAPII in K562 and HepG2 cell lines and then mapped to different parts of genes with GC exons or AT exons. d Box plots of the mean coverage by RNAPII of GC exons and AT exons. “***” corresponds to Wilcoxon’s test P < 10−6. e Density of reads obtained after immunoprecipitation of RNAPII phosphorylated at serine 2 (RNAPII-ser2) in K562 and HepG2 cell lines and then mapped to different parts of genes with GC exons or AT exons. f Box plots of the mean coverage of phosphorylated RNAPII at GC exons or AT exons. “***” corresponds to Wilcoxon’s test P < 10−6