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Fig. 8 | Genome Biology

Fig. 8

From: VULCAN integrates ChIP-seq with patient-derived co-expression networks to identify GRHL2 as a key co-regulator of ERa at enhancers in breast cancer

Fig. 8

Effect of GRHL2 knockdown after 24 h on eRNA at E2-responsive binding sites and overexpression of GRHL2 Δ425–437. (a) Overexpression of GRHL2 in MCF7 resulted in a reduction of eRNA transcribed from the GREB1, TFF1, and XBP1 enhancers. The effect was significant at TFF1 and XBP1 enhancers (p < 0.05, paired t test). (b) Overexpression of GRHL2 Δ425–437 (delta) compared to empty vector (EV) and GRHL2 wild type (OE) at 24 h. In all three cell lines at all three loci, overexpression of the wild type (WT) led to a reduction in the mean eRNA production at GREB1, TFF1, and XPB1. This effect was significant in six out of nine experiments (p < 0.05, t test, one-tailed, paired). Overexpression of GRHL2 Δ425–437 had a reduced effect that led to a significant reduction in only two out of nine experiments (p < 0.05, t test, one-tailed, paired). Importantly, in four out of nine experiments, WT overexpression had significantly less eRNA production than GRHL2 Δ425–437, suggesting the P300 inhibition domain plays a role in the regulation of eRNA production

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