Fig. 4
From: Plant lamin-like proteins mediate chromatin tethering at the nuclear periphery
Loss of specific chromatin positioning at the nuclear periphery in crwn mutants. a Design of dual-color FISH probes targeting a 10-Mb region in chromosome 1 according to NUP1:GFP RE-ChIP results. Green and red rectangles depict BACs. Chromatin regions showing preference of staying closer to the NP (having higher RE-ChIP signals) are labeled as Green probes. b Representative confocal images of wild-type and mutant 2C nuclei. The plots on the right hand side show cumulative chromosome painting signal as a function of the distance to the NP. P0.5 denotes the percentage of signals found within 0.5 μm from the NP. c Comparison of chromosome painting signals (P0.5) in different plants. The P0.5 values of Green and Red probes of each nucleus are connected by a blue or red line, indicating whether P0.5(Green) is larger or not, respectively. G and R, Green and Red probes as indicated in a, respectively. p values mean results of Wilcoxon signed-rank tests