Fig. 3
From: CRISPR directed evolution of the spliceosome for resistance to splicing inhibitors
SGRs confer resistance to GEX1A treatment and the structural basis of resistance. a Dose–response effects of GEX1A treatment on germination of WT, SGS, SGR1, SGR2, SGR3, SGR4, SGR5, and SGR6 seeds. Rice seeds were sterilized and germinated in dH2O with different concentrations of GEX1A. Hypocotyl emergence was considered as germination. We observed that the rice seed germination was affected in a dose-dependent manner (Additional file 1: Figure S1). Germination of wild-type and SGS1 seeds was severely inhibited at 2.5 and 5 μM GEX1A. Germination of SGRs is less affected by GEX1A treatment. SGR4 germination is completely unaffected even at 10 μM GEX1A (n = 8). b The structural basis of the SF3B1 mutant variant resistance to GEX1A. The 3D model for the OsSF3B1:PHF5A complex and GEX1A with OsSF3B1 is represented in gray, PHF5A is represented in cyan, and GEX1A represented in blue. Key residues on PHF5A are shown in orange whereas residues for wild type are shown in magenta and mutations on OsSF3B1 are shown in gray