Fig. 2

Evaluation of CRISPR/Cas9 expressing transgenics for geminivirus resistance. a Infection rates of cassava mosaic disease symptoms on agro-inoculated plants monitored weekly over an 8-week period. A minimum of five independent replicates per transgenic lines were infected. b Virus levels in symptomatic plant samples at 3 and c 8 weeks post infection (wpi). Levels were measured on three replicated pools of symptomatic leaves from a minimum of five individual plants per line. (ns = no statistical significance observed using Dunn’s multiple comparisons test). d, e %identity of each virus sequence per line, measured against the reference sequence, across a 100-nt window surrounding the sgRNA site and across almost the entire viral genome respectively