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Table 4 Validated pathogen identifications in patients with neurological infections have high numbers of unique k-mers per read. The pathogens were identified with as few as 15 reads, but the high number of unique k-mers indicates distinct locations of the reads along their genomes. Re-alignment of mapped reads to their reference genomes (column “Covered bases”) corroborates the finding of the unique k-mers (see also Additional file 1: Figure S4). Interestingly, the k-mer count in PT5 indicates that there might be multiple strains present in the sample since the k-mers cover more than one genome. Read lengths were 150–250 bp

From: KrakenUniq: confident and fast metagenomics classification using unique k-mer counts

Sample

Matched microorganism

Reads

k-mers

Covered bases

PT5

Human polyomavirus 2

9650

7129

5130/5130

PT7

Elizabethkingia genomo sp. 3

403

20,724

53,256/4,433,522

PT8

Mycobacterium tuberculosis

15

1570

2227/4,411,532

PT10

Human gammaherpesvirus 4

20

2084

2822/172,764