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Fig. 5 | Genome Biology

Fig. 5

From: LaminA/C regulates epigenetic and chromatin architecture changes upon aging of hematopoietic stem cells

Fig. 5

LaminA/C expression in the hematopoietic system is specific to HSCs and is decreased upon aging. a Representative confocal 3D reconstruction of the nucleus (stained with DAPI, blue) and LaminB (green) of young, aged, and aged+CASIN 5 μM HSCs. Bar = 2 μm. b Representative confocal 3D reconstruction of the nucleus (stained with DAPI, blue) and of LaminA/C (green) of young, aged, aged+CASIN 5 μM HSCs, and young ST-HSCs. Bar = 2 μm. c Lmna transcript levels in young, aged, aged+CASIN 5 μM HSCs, and in young ST-HSCs. Shown are mean values + 1 SE; n = 3 biological repeats; ****p < 0.0001. d Representative distribution of LaminA/C (green) in young HSCs. Bar = 2 μm. The arrow indicates LaminA/C distribution in the deep invagination of young HSCs nuclei. e Percentage of young HSCs with invaginated nuclei, n = 4 biological repeats, 45 single HSCs in total. f Percentage of young HSCs which have LaminA/C distribution in the invagination site of HSCs nuclei out of invaginated HSCs, n = 4 biological repeats, 45 single HSCs in total. g Representative confocal 3D reconstruction of the distribution of H3K9me2 in young, aged, and aged CASIN-treated LT-HSCs. Bar = 2 μm. h Percentage of young, aged, and aged+CASIN 5 μM HSCs presenting with peripheral heterochromatin localization of H3K9me2, n = 3 biological repeats, ~ 150 single HSCs for young and aged, 20 single HSCs for aged+CASIN 5 μM in total; *p < 0.05. i Representative FACS dot plots of B220+, CD3+, and myeloid (Gr1+, Mac1+, and Gr1+Mac1+) cells in control Lmnafl/fl and LmnaΔ/Δ/Vav-Cre PB. j Percentage of B220+, CD3+, and myeloid (Gr1+, Mac1+, and Gr1+Mac1+) cells in PB of control Lmnafl/fl and LmnaΔ/Δ/Vav-Cre mice; *p < 0.05, n = 4–6 mice. k Hematologic analyses of control Lmnafl/fl and LmnaΔ/Δ/Vav-Cre mice. *p < 0.05, **p < 0.01; n = 5–6 mice

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